NaturegenicPlatform TechnologyCRISPR Gene Editing
Platform Technology

CRISPR gene editing technology is a power tool for editing genomes, which originated from an antiviral defense system of bacteria. CRISPR stands for “clusters of regularly interspaced short palindromic repeats.” CRISPR gene editing technology uses RNA-guided endonucleases to precisely locate and cut target nucleic acid sequences. The RNA-endonuclease complex consists of CRISPR-associated protein, Cas, that binds and cleaves nucleic acids, and a strand of RNA that guides Cas to the exact location in genome.

CRISPR Genome Editing Technology

Next generation genome editing technology through advanced of CRISPR system

CRISPR PLUS™ Gene Editing Design
image

▶ Add protein functionality to improve performance

Efficiency Comparison
image

▶ In difficult-to-edit stem cells, efficiency is increased by up to 15 times

gfCas12a™ Genome Editing
(Patent Register)

Improvements in gene editing efficiency and accuracy

Compared to the existing Cas9,

Up to 10 times higher indel efficiency

Compared to the existing Cas9,

Up to 2 times higher Knock-in efficiency

Compared to the existing Cas9 in Tobacco protoplasts,

indel efficiency increased about 3 times Reagent
(Purchase Order)
gfCas12a™ gene editing special feature
image

▶ Improved performance by reorganizing amino acid sequence and adding signal peptide

Efficiency Comparison
image

▶ Able to edit the area in DNA that Cas9 cannot : up to 90% increase in editing efficiency

CRISPR PLUS™ Genome Editing
(Patent Register)

More accurate and target-specific genonme editing technology than CRISPR/Cas9

In vitro DNA cleavage efficiency experiment, collateral DNase activity Greatly Reduced

Indel (insertion/deletion) efficiency in plant cells Up to 7 times improved

When combined with enhancers, gene editing efficiency is greatly improved by up to 90%

Reagent
(Purchase Order)